Protoplast-derived calli were subcul-tured on the MSB agar medium with 0. 2 mg/L 2, 4-D and 0. 1 mg/L 6-BA and then transferred onto MSB medium containing 2. 0 mg/L 6-BA to induce shoots.

• 该愈伤组织在附加2；4-D 0.；2mg/L、6-BA 0