Primary MSCs covering the bottom of culture bottle were washed once with D-Hanks liquid,and digested for 4 minutes with 0.25% trypsin. Then LG-DMEM was used to collect and deposit the cells at 1 000 r/min for 10-minute centrifugation.

• 兔骨髓间充质干细胞的克隆化:将铺满培养瓶底的原代骨髓间充质干细胞；用D-Hanks液小心的洗1次；加入0.；25%25胰蛋白酶消化约4min；弃消化液；加LG-DMEM培养液收集细胞；1000r/min；离心10min；然后用LG-DMEM培养液充分混匀沉淀细胞。