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Porcine parvovirus(PPV) was cultivated on IBRS-2 cell, after CPE was observed, the PPV was harvested, frozen and thawed, then inactivated with Formalin, precipitated with (NH4 )2S4., finally dialysed and concentrated.
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将猪细小病毒在IBRS-2细胞上同步培养增殖,待出现细胞病变后,反复冻融,收获病毒液,用甲醛灭活。
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