Partial rag1 and full length of rag2 were recombined and fused on plasmidpPROEX~(TM) HTa, and was transferred into E. coli (BL-21) for RAG1 and RAG2expression.

• 将牙鲆rag1基因的一部分(编码559个氨基酸)和rag2全基因序列(编码533个氨基酸)插入到表达质粒pProEXTM HTa上；在大肠杆菌E.