PCR. The MDK promoter/enhancer (MDKp) sequence with runt domain binding sites was amplified from the human genome DNA and directionally cloned into pGL3-Basic Luciferase reporter vectors to construct the MDKp-pGL3-B expression plasmids.

• 从基因组DNA中PCR扩增一段含Runt结合顺序的MDK基因启动子/增强子序列(MDKp)，定向克隆到pGL3-Basic质粒，构建MDKp-pGL3-Basic Luciferase报告基因表达载体；