Once a plasmid is snipped open, a foreign piece of DNA, cut by the same enzyme scissors, can be taped, end to end, into the plasmid using another enzyme, DNA ligase.

• 一旦原生质被分开后，用同样限制酶剪开的外来DNA片段和它的被切断的端口被粘到一起，形成了新的原生质。连接时使用的是另一种酶--DNA连接酶。