Objective:To establish a hyperinsulinemic euglycemic clamp technique in conscious rat,and to explore the effect of anesthesia and acute infusion of lipid on insulin sensitivity of rats.

• 目的:建立清醒状态下大鼠高胰岛素-正常血糖钳夹术,并探讨短期输注乳化脂肪和手术后麻醉状态对大鼠胰岛素敏感性的影响。方法:采用颈动、静脉插管术。