Objective: To develop two methods for determining the contents of phenobarbital in serum by differential spectrophotometry under two conditions,to comparison the average recovery,BSD,stability and noise of signal of determination et.

• 目的:建立两种条件下差示分光光度法测定血清苯巴比妥浓度的方法,比较其回收率、RSD、稳定性和信号响应等。