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Multiplex PCR results demonstrated that all strains had the expected specific band of 324 bp cpa gene fragment of C. perfringens, but specific cpb, etx and iA gene fragment did not appear. Thus all these strains can be classified as type A of C.
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经多重PCR方法鉴定,均扩增出预期大小为324bp的cpa基因片段的特异性条带,未扩增出针对cpb、etx和iA基因的特异性条带,证实均为A型产气荚膜梭菌。
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