Methods:Triton/tris was used to extract the crude H 2,to which of the anti H 2 antibodies coupled with sepharose 4B was applied to purify H 2 antigen by affinity chromatography. The coupled H 2 Ag was eluted via 0.5%DOC,0.65M NaCl solution.

• 用0.；5%25Triton/Tris抽提小鼠组织相容性抗原（H－2）抗原，利用抗H－2抗原抗体制备的亲和柱，特异性结合H－2抗原，再用0