Methods:The HIF-dependent recombinant adenovirus Ad-5HRE/hCMVmp-BCD was constructed by DNA recombinant technique. Western blot was used to detect HIF-dependent expression of bacterial cytosine deaminase (BCD).

• 方法借助DNA重组技术构建HIF依赖性表达的重组腺病毒载体Ad-5HRE/hCMVmp-BCD。