Methods:Rats cerebella granule neurons were pre incubated with chorea toxin or dibutyryl cAMP for 20 min to raise the intracellular cAMP levels,and then cultured on the immobilized myelin substrate.

• 方法 :用霍乱毒素、双丁酰环磷腺苷预先孵育小脑颗粒神经元以间接和直接提高胞内cAMP ,培养神经元于髓鞘膜蛋白基质上 ,观察对神经元突起生长的影响。