Methods: Vectors expressing Green Fluorescent Protein- Fibrous Actin (GFP-F-Actin) and F-GFP were co-transfected into cultured rat hippocampal neurons at 5 d in vitro (DIV 5).

• 方法:出生后第1天进行海马神经元培养,于第5天转染绿荧光蛋白(GFP)标记的纤维型肌动蛋白(GFP-F-Actin)和定位在膜上的GFP(F-GFP),观察培养第7~14天树突的发育情况并进行量化分析。