Methods: Polymerase chain reaction-restrictive fragment length polymorphism (PCR-RFLP) was used to detect the MTHFR gene C677T polymorphism in 53 normal controls and 182 infertile males.

• 方法：应用聚合酶链式反应-限制性片断长度多态性(PCR-RFLP)方法检测53例健康可育男性和182例不育男性的MTHFR基因C677T位点多态性。