Methods: Methylation-specific PCR(MSP) was used to detect the frequency of aberrant methylation of RASSF1A and p16 gene in 96 human NSCLC tissues and matched 96 normal lung tissues far from cancer area.

• 方法:用甲基化特异性PCR(MSP)方法对96例NSCLC患者癌组织及相应的远癌正常肺组织中RASSF1A和p16基因启动子甲基化状态进行检测。