Methods: 1.We collected Leishmania promastigotes,washed its with 0.01mol/LpH7.2 buffers ,smashed its,and then measured protein content and titrated work concentration(by ELISA).

• 方法:1、抗原的提取:收集新疆不同地区分离得到的利什曼原虫前鞭毛体；经培养；用0.；01mol/L pH7