Methods Using plasmid as template,full-length EGFP gene was amplified. The amplified EGFP gene and pET28a vector were cleaved by EcoR I and Hind III,then ligated after purifying.

• 方法以质粒pEGFP-N1为模板扩增EGFP基因,EcoR I和Hind III双酶切EGFP基因序列和pET28a载体。