Methods The rearrangements of AML1 and ETO genes were detected by Southern Blot and the AML1-ETO fusion gene by nested RT-PCR combined with sequencing in K562 cells treated with etopside (Vp16) and doxorubicin(DOX).

• 方法 K5 6 2细胞经不同浓度足叶乙甙 (Vp16 )和阿霉素 (DOX)作用不同时间后用Southern杂交检测AML1、ETO基因重排 ,用筑巢式RT PCR联合序列分析检测AML1 ETO融合基因。