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Methods The rat osteoblasts were isolated and cultured by enzyme digestion, and edherent cell analysis andsorting cytometer (ACAS) was employed to assay the change of intracellular Ca2+ concentration of rat osteoblasts.
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方法酶消化法体外分离培养胎儿鼠成骨细胞,应用ACAS检测成骨细胞内Ca2+脓度的变化。
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