Methods The primer of LT gene from Enterotoxigenic E.coli was designed and the condition of PCR was optimized to identify ETEC with PCR amplification for 16 samples.

• 方法设计肠毒素大肠杆菌LT基因的特异性引物,优化PCR反应条件,对16种样品进行细菌通用引物和特异性引物的PCR扩增,从而鉴定肠毒素大肠杆菌。