Methods The cells from the embryonic human cortex were mechanically dissociated.The N2 medium was adopted to culture and expand the cells which were identified by immunocytochemistry.

• 方法 从胎脑皮质中机械分离细胞，N2培养基培养和扩增人类神经干细胞，应用免疫细胞化学方法对培养的细胞进行鉴定。