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Methods The antibodies to ouabain were prepared by immunizing rabbits.The optimum antisera titer and concentration of coating buffer with chessboard test and the assay were established.
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方法利用外源性哇巴因免疫家兔制备哇巴因抗血清,用棋盘试验确定抗血清的稀释度及包被抗原的浓度;
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