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Methods Survivin-targeting shRNA eukaryotic expression vector pRNAT-U6. 1/neo-survivin was constructed and identified. Human bladder cancer cell T24 was transfect-ed with pRNAT-U6. 1/neo-survivin and then stable monoclonal cells were selected.
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方法构建Survivin基因shRNA真核表达载体pRNAT-U6.;1/neo-survivin;转染人膀胱癌细胞T24并获得稳定的单克隆细胞。
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