Methods Semi-quantitative RT-PCR was used to detect the expression of visfatin mRNA in omental and abdominal subcutaneous adipose tissue of 22 cases with type 2 diabetes and 20 cases of non-diabetes controls.

• 方法用半定量RT-PCR方法检测糖代谢正常者(对照组20例)和T2DM患者(22例)的大网膜与皮下脂肪组织visfatin mRNA的表达水平,并测量体重、血压、腰围、臀围、血糖、空腹胰岛素、血脂和HOMA-IR。