Methods Realtime fluorescent quantitative RT-PCR was used to detect the CK20 mRNA expression in the portal venous blood of 31 patients with colon carcer and 20 healthy volunteers.

• 方法采用实时荧光定量RT-PCR检测31例病理学确诊的结肠癌患者门静脉血和20例健康对照者外周血CK20mRNA的表达水平。