Methods Rate of formation of P-nitroaniline was determined by the rate of increase in absorbance at 405 nm.

• 方法LAP可催化L-亮氨酸-P-硝基苯胺水解生成L-亮氨酸和P-硝基苯胺,测定405 nm处P-硝基苯胺吸光度的增加速度,求得LAP活性。