Methods Peripheral blood mononuclear cells(PBMCs) were stained by monovlonsl antibody,and changed proportion of CD4+CD25+FOXP3+ T regulate cells were determined by Flow cytometer.

• 方法用荧光标记的单克隆抗体染色外周血单个核细胞,利用流式细胞仪检测CD4+CD25+FOXP3+调节性T淋巴细胞的改变。