Methods Ordinary PCR, Southern blot and direct ISPCR were applied and compared in detecting Hib in 100 paraffin-embedded lung tissues of autopsy children died of CAP.

• 方法用普通PCR、Southern杂交和直接ISPCR三种方法检测100例死于肺炎的儿童尸检石蜡包埋肺组织标本中的Hib,分析其致病、致死及在肺组织中的分布情况,并对这些方法对比分析。