Methods KG_1 was stimulated by phorbol ester(PMA) in combination with ionomycin. Expression of DC-SIGN was detected by western blotting, RT-PCR, and flow cytometry in KG_1 and KG_1 derived DLCs of different stimulation periods.

• 方法采用佛波酯(PMA)和离子霉素(Ionomycin)诱导KG1细胞为DLCs,采用Western免疫印迹、RT-PCR和流式细胞仪检测KG1和KG1来源的DLCs不同诱导时期DC-SIGN的表达。