Methods Immunohistochemical technique was used to study NTFs genes expression degree at several time points after stereotaxical electrolytic injury within the region of bulbar respiratory center.

• 方法实验动物采用SD大鼠，以直流电解损毁法建立一侧延髓呼吸中枢损伤动物模型，采用免疫组化染色法对损伤后不同时间点NTFs基因的表达特点进行了研究。