Methods HSP70 cDNA was inserted into polylinker sitr of eukaryotic expression vector pcDNA 3.1 to construct pcDNA AHSP70, in which restriction enzyme analysis was used to confirm the reverse orientation of the HSP70 cDNA from the individual transformants.

• 方法 将HSP70cDNA反向克隆入 pcDNA 3.；1；构建CMV启动子控制的真核表达载体 pcDNA AHSP70；用酶切鉴定结果 ;