Methods Expression of perforin and Fas L of TIL was studied in 20 HCC cases using in situ hybridization and immunohistochemistry. Results Expression of perforin and Fas L gene were detected in TIL in 80% of the cases studied.

• 方法应用原位杂交及免疫组化ABC方法检测20例病人原发性肝细胞癌组织的TIL中穿孔素及Fas?L的mRNA及其蛋白的表达情况。