Methods Accumulation of ciprofloxacin in clinical isolates of Escherichia coli was measured by fluorometry, and acrAB gene was identified by PCR and Southern blot.

• 方法 应用荧光法测定临床分离大肠杆菌对环丙沙星的主动外排功能 ; PCR及Southernblot测定主动外排系统AcrAB结构基因acrAB ;