Methods A549 cells were treated with different cytotoxic drugs for 24hours. The changes of APE/ref-1 expression in A549 after drug-treatment were detected by real time PCR, western blot and streptavidin peroxidase immunohistochemistry staining.

• 方法 通过免疫组化染色明确APE/ref-1在A549细胞中的表达后,以特定浓度的临床常用细胞毒性药物对A549细胞进行诱导,采用实时荧光定量PCR、Western Blot和免疫组化技术检测药物诱导前后A549细胞内APE/ref-1表达的变化情况。