Methods 30 ampuls in the experiment group were opened by sterilized tweezers.The other 30 ampuls in control group were opened by hand.Liguid in each ampul was cultiuated immediately.

• 方法将消毒瓶镊折断安瓿方法为实验组,将手折断安瓿方法为对照组,各折断安瓿30支后进行药液培养。