Methods 20 loci on chromosome 9 were examined to detect loss of heterozygosity(LOH) in 21 cases of glioblastoma by PCR based microsatellite polymorphism analy-ses, in which fluorescence-labeled primers and Perkin Elmer 377 DNA Sequencer were applied.

• 方法 应用聚合酶链反应（PCR）方法，采用荧光标记引物和377型DNA序列自动分析仪，分析21例 GBM 9号染色体上 20个微卫星多态性标记的LOH。