Method: One episomal HPV16 CTSE and one wild-type HPV16 CTSE fragment were amplified with PCR and inserted into pUCm-T vector respectively, following pUCm-T vector reference.

• 方法：本实验选取游离型HPV16阳性的宫颈癌组织（已测序证实存在变异的CTSE片段）及野生型HPV16质粒DNA为模板，PCR扩增CTSE片段，克隆到中间载体pUCm-T，酶切、PCR、测序等方法鉴定插入成功。