Method: (1) NS3 protease gene and single chain NS3/4A gene were both subcloned into pQE-30 and expressed in E.

• 方法:(1) 用原核表达载体pQE-30分别构建含有NS3蛋白酶基因的pQE/NS3和含有单链NS3/4A基因的pQE/NS3/4A重组质粒;