Mediated by PMS, the NADH resulting from the reaction catalysed by MDH isozymes can reduce K 3Fe(CN) 6 into K 4Fe(CN) 6. Then K 4Fe(CN) 6 can react with FeCl 3 resulting in Prussian blue which dyes MDH isozymes.

• 以N -甲基吩嗪甲基硫酸盐 (PMS)为介体 ,使苹果酸脱氢酶 (MDH)同工酶催化反应产生的NADH将铁氰化钾还原为亚铁氰化钾 ,再利用亚铁氰化钾与三氯化铁反应生成普鲁士蓝的性质 ,实现对苹果酸脱氢酶同工酶进行染色的目的