Meanwhile, the feline IL-18 gene was subcloned into the prokaryotic expressing vector pET28a and recombinant vector was further transformed into Escherichia coli strain BL21(DE3) for expression under the induction of IPTG.

• 将目的基因片段进一步亚克隆到大肠杆菌(Escherichia coli)表达载体pET28a中构建了重组质粒pETIL-18,转化大肠杆菌BL21(DE3),并用IPTG诱导。