Many recombinant proteins fail to fold into native state with activity when expressed in Escherichia coli,instead,they often misfold and are easy to accumulate as inclusion bodies.

• 重组蛋白技术在许多领域已成为一种必要的工具,但有时表达的目的蛋白却未进行正确的折叠而被蛋白酶降解或聚集形成包涵体。