MTT, PNPP and ARS were used to observe the proliferation,activity of ALP and the number of mineral node of cultured osteoblasts in vitro.

• 应用MTT法,对硝基苯磷酸盐法(PNPP)及茜素红染色方法观察补肾活血方对体外培养成骨细胞的增殖、碱性磷酸酶(ALP)活性及矿化结节形成的影响。