METHODSThe hydroxycamptothecin liposomes were prepared by thin film method with freeze-thawing steps,Unloaded hydroxycamptothecin was separated by Sephadex-G50 chromatography.

• 方法:采用薄膜分散-冻融法制备羟基喜树碱脂质体;用凝胶柱色谱法分离游离药物;高效液相色谱法测定包封率。