METHODS:The islets were isolated from NMRI mice by collagenase and DNase digestion. Millipore multiscreen assay system was used to observe the effects of PACAP 38 on the function of isolated islets.

• 方法:用胶原酶和DNA酶联合消化法分离NMRI小鼠胰岛,RPMI1640组织培养液过夜培养,采用MilliporeMultiscreen系统观察不同浓度PACAP-38对胰岛功能的影响。