METHODS:Radix Polygonum Muhiflori was steamed with black bean sauce for 2,4,6,8,10 h,respectively. The contents of nomadic and total anthraquinone were determined by the magnesium acetate colorimetry directly and after acidolysis,respectively.

• 方法何首乌分别用黑豆汁拌蒸2、4、6、8、10h,采用直接醋酸镁比色法和酸水解后醋酸镁比色法,分别测定炮制饮片中游离蒽醌和总蒽醌的含量。