METHODS: Eukaryotic expression vectors pTL 8/apical membrane antigen 1 (AMA 1) (tTA) and pTL 8/AMA 1(rtTA)gene which express trans activator (tTA) or reverse trans activator(rtTA), respectively, and AMA 1 gene of Plasmodium falciparum were constructed.

• 方法 :首先构建恶性疟原虫顶端膜抗原 1(AMA 1)基因和转录激活因子 (tTA或rtTA)基因的真核表达质粒pTL 8/AMA 1和pTL 8/AMA 1(rtTA) ,并大量制备这两种质粒及表达转录抑制子 (tTS)的质粒pUHS6 1。