METHODS: Bone marrow blood was sterilely collected.Human BMSCs were cultured in monoclone by density gradient centrifugation and adherence screening.Cells of 90% confluency were digested by trypsin.

• 方法：无菌条件下抽取骨髓血，联合应用密度梯度离心法及贴壁筛选法，以单克隆形式分离培养人骨髓间充质干细胞，当细胞汇合至90%25时胰蛋白酶消化传代。