METHODS Recombinant eukaryotic expression vector pcDNA3.1 S0.7 was constructed by cloning S segment 0.7 kb fragment into pcDNA3.1 (+) and transfected COS 7 cells by lipofectin. Immunoflourescence was used to identify the expression.

• 方法 构建汉坦病毒 76 - 118株 S基因 5 端 70 0 bp片段的真核表达载体 pc DNA3.；1- S0