METHODS Portal vein cannulation and perfusion combined with collagenase digestion were used to isolate pig pup hepatocytes.They were then cultured and passaged into the conditioned medium.

• 方法 用门静脉插管原位灌注结合胶原酶消化法分离乳猪肝细胞；应用条件培养基对其进行体外培养；观察其形态、增殖、分化和功能等方面的变化.