METHODS Dicyclohexylcarbodiimide(DCC) was used to conjugate FA to bovine serum albumen(BSA) and then the quantity ratio of the two reactants was changed to get three different antigens.

• 方法采用二环己基碳二亚胺法将阿魏酸偶联到牛血清白蛋白,选择FA与BSA的不同比例,合成3种结合比不同的人工抗原。